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Volume 30, Number 4—April 2024
Research

Divergent Pathogenesis and Transmission of Highly Pathogenic Avian Influenza A(H5N1) in Swine

Bailey ArrudaComments to Author , Amy L. Vincent Baker, Alexandra Buckley, Tavis K. Anderson, Mia Torchetti, Nichole Hines Bergeson, Mary Lea Killian, and Kristina Lantz
Author affiliations: Agriculture Research Service, Ames, Iowa, USA (B. Arruda, A.L. Vincent Baker, A. Buckley, T.K. Andersen); Animal and Plant Health Inspection Service, Ames (M. Torchetti, N. Hines Bergeson, M.L. Killian, K. Lantz)

Main Article

Figure 7

Immunohistochemical detection of influenza A virus nucleoprotein antigen in swine infected with H5N1 highly pathogenic avian influenza belonging to the goose/Guangdong 2.3.4.4b hemagglutinin phylogenetic clade. A) Extensive labeling of pneumocytes lining alveolar septa (arrows) and respiratory epithelium lining bronchioles (arrowheads) in the lung of pig 794 infected with A/bald eagle/FL/22 necropsied at 3 days postinoculation (dpi). Hematoxylin & eosin stain; original magnification ×40. B) Extensive labeling of pneumocytes lining alveolar septa (arrow), respiratory epithelium lining a bronchus (arrowheads), cell membrane of alveolar macrophages (chevron), and within the cytoplasm and nucleus of alveolar macrophages consistent with viral replication (notched arrow) in the lung of pig 798 infected with A/bald eagle/FL/22 necropsied on 5 dpi. Hematoxylin & eosin stain; original magnification ×40. C) Labeling in the cytoplasm (arrows) and nucleus (arrowhead) of endothelial cells in the lung of pig 791 infected with A/bald eagle/FL/22 necropsied on 3 dpi. Hematoxylin & eosin stain; original magnification ×200. D) Labeling of respiratory epithelium lining a bronchus (arrowheads), within the cytoplasm and nucleus of alveolar macrophages consistent with viral replication (notched arrow), rarely pneumocytes (arrow), in the lung of pig 58 infected with A/raccoon/WA/22 necropsied on 3 dpi. Hematoxylin & eosin stain; original magnification ×40. E) Abundant labeling of respiratory epithelium lining a bronchiole (arrowheads) and within the cytoplasm and nucleus of alveolar macrophages consistent with viral replication (notched arrow) in the lung of pig 78 infected with A/redfox/MI/22 necropsied on 3 dpi. Hematoxylin & eosin stain; original magnification ×100.

Figure 7. Immunohistochemical detection of influenza A virus nucleoprotein antigen in swine infected with H5N1 highly pathogenic avian influenza belonging to the goose/Guangdong 2.3.4.4b hemagglutinin phylogenetic clade. A) Extensive labeling of pneumocytes lining alveolar septa (arrows) and respiratory epithelium lining bronchioles (arrowheads) in the lung of pig 794 infected with A/bald eagle/FL/22 necropsied at 3 days postinoculation (dpi). Hematoxylin & eosin stain; original magnification ×40. B) Extensive labeling of pneumocytes lining alveolar septa (arrow), respiratory epithelium lining a bronchus (arrowheads), cell membrane of alveolar macrophages (chevron), and within the cytoplasm and nucleus of alveolar macrophages consistent with viral replication (notched arrow) in the lung of pig 798 infected with A/bald eagle/FL/22 necropsied on 5 dpi. Hematoxylin & eosin stain; original magnification ×40. C) Labeling in the cytoplasm (arrows) and nucleus (arrowhead) of endothelial cells in the lung of pig 791 infected with A/bald eagle/FL/22 necropsied on 3 dpi. Hematoxylin & eosin stain; original magnification ×200. D) Labeling of respiratory epithelium lining a bronchus (arrowheads), within the cytoplasm and nucleus of alveolar macrophages consistent with viral replication (notched arrow), rarely pneumocytes (arrow), in the lung of pig 58 infected with A/raccoon/WA/22 necropsied on 3 dpi. Hematoxylin & eosin stain; original magnification ×40. E) Abundant labeling of respiratory epithelium lining a bronchiole (arrowheads) and within the cytoplasm and nucleus of alveolar macrophages consistent with viral replication (notched arrow) in the lung of pig 78 infected with A/redfox/MI/22 necropsied on 3 dpi. Hematoxylin & eosin stain; original magnification ×100.

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Page created: March 07, 2024
Page updated: March 20, 2024
Page reviewed: March 20, 2024
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