Key points
- PulseNet was established in 1996,
- The mission of PulseNet is to connect foodborne illness cases together using the DNA 'fingerprints' of the bacteria making people sick in order to detect outbreaks.
Overview
PulseNet is a national laboratory network that connects foodborne, waterborne, and One Health–related illness cases to detect outbreaks. PulseNet uses the DNA fingerprints of bacteria making people sick to detect thousands of local and multistate outbreaks. Since the network began in 1996, PulseNet has improved food safety systems through identifying outbreaks early. This allows investigators to find the source, alert the public sooner, and identify gaps in food safety systems that would not otherwise be recognized. PulseNet International performs a similar role for foodborne illnesses globally.
- National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of Foodborne, Waterborne, and Environmental Diseases (DFWED)
- Next Generation Technology [PDF - 1 page]
- Whole Genome Sequencing (WGS) Workflow [PDF - 1 page]
- Standard operating procedure for WGS sequencing on MiSeq [PDF - 20 pages]
- Standard operating procedure for PulseNet total DNA extraction and quality control of purified DNA extracts [PDF - 21 page]